What is Tris-HCl Buffer, pH 7.0 used for?

Tris-HCl Buffer, pH 7.0 is commonly used in biological and biochemical applications. It helps to maintain a stable pH environment that is essential for enzyme activity, DNA/RNA electrophoresis, and protein purification. The Tris buffer effectively minimizes pH fluctuations, allowing researchers to achieve more reproducible results in experiments. For example, in molecular biology experiments, a stable pH is crucial during reactions such as PCR or gel electrophoresis, where variations could lead to distorted or inaccurate results.

How do you prepare Tris-HCl Buffer at pH 7.0?

To prepare Tris-HCl Buffer at pH 7.0, you need to dissolve Tris base in distilled water and adjust the pH with hydrochloric acid (HCl) to the desired level. The buffering capacity will depend on the concentration of Tris and the final volume. For practical purposes, pre-made 1M solutions like Tris-HCl Buffer, pH 7.0, provide convenience and consistency, especially in labs where precise pH values are critical. This method is routinely applied in research facilities for applications that require consistent buffering capacity.

Can Tris-HCl Buffer be used in protein assays?

Yes, Tris-HCl Buffer, pH 7.0, is widely used in protein assays. Its neutral pH is compatible with many proteins, which ensures their stability during analysis. For instance, it is remarkable for techniques like Western blotting and ELISA, where protein stability and enzymatic activity are paramount. Using the buffer prevents denaturation and maintains the structural integrity of proteins throughout the assay process, making it a go-to solution for researchers working on enzyme-linked studies.

Is Tris-HCl Buffer suitable for electrophoresis?

Absolutely! Tris-HCl Buffer, pH 7.0, is specifically formulated for electrophoresis applications. The buffer provides optimal conditions for the migration of DNA and RNA through gel matrices during electrophoretic techniques. It also helps to maintain the stability of nucleic acids, ensuring that they do not degrade during the process. Often, labs rely on this buffer in both agarose and polyacrylamide gel electrophoresis, where a consistent pH is crucial for resolving nucleic acid fragments accurately.

What volume of Tris-HCl Buffer should I use in experiments?

The volume of Tris-HCl Buffer, pH 7.0, to use in experiments varies depending on the specific protocol you are following. Commonly, for reactions and assays, volumes of 50-200 µL are typically sufficient for small-scale experiments, while larger setups may require several milliliters. By referencing the specific requirements of your experiment, you can ensure you are using the appropriate buffer volume for optimal results, supporting consistent outcomes throughout various laboratory applications.

How should Tris-HCl Buffer be stored?

Tris-HCl Buffer, pH 7.0, should be stored at room temperature or in a cool place and kept tightly sealed to prevent contamination. It's advisable to protect it from extreme temperatures or direct sunlight. Under these conditions, the buffer can be stored for extended periods while maintaining its stability. This practice is particularly important in laboratory settings where consistent results are needed, ensuring that the buffer maintains its pH and efficacy for future experiments.

Can Tris-HCl Buffer be used for DNA or RNA storage?

Tris-HCl Buffer, pH 7.0, can indeed be used for storing DNA or RNA samples, as it helps to prevent degradation by providing a stable pH environment. Storing nucleic acids in this buffer allows for long-term preservation without compromising their integrity, making it ideal for sample management in molecular biology. This usage is especially beneficial in lab settings when preparing samples for further analysis or experimentation, where maintaining their quality is paramount.

What are the benefits of using a pre-made Tris-HCl Buffer solution?

Using a pre-made Tris-HCl Buffer, pH 7.0 streamlines laboratory processes by ensuring correct concentrations and pH values are achieved without requiring additional calculations or adjustments. This saves time and reduces the possibility of error in preparation, which is critical for experiments demanding precision. Pre-made buffers are particularly valuable in high-throughput scenarios or collaborative research, where multiple scientists rely on the same consistent buffer for their methodologies.

What are the common applications of Tris-HCl Buffer in research?

Tris-HCl Buffer, pH 7.0, is versatile and finds applications in various domains, such as molecular biology, biochemistry, and genetic research. Common uses include enzyme assays, protein purification, PCR, and nucleic acid gel electrophoresis. Its ability to maintain consistent pH makes it an essential reagent during experiments that demand precision, thus facilitating accurate interpretation of results and enhancing reproducibility across experiments or research laboratories.

Where can I buy Tris-HCl Buffer, pH 7.0 [1M] - 250mL?

You can buy Tris-HCl Buffer, pH 7.0 [1M] - 250mL from Ubuy in Croatia. Ubuy offers a convenient platform for purchasing various laboratory supplies, including specialized buffers like Tris-HCl. Their inventory ensures that you can find quality products delivered directly to your location, catering to the needs of researchers and laboratory professionals looking for reliable reagents.

Kako kupovati Tris-HCl Buffer, pH 7.0 [1M] - 250mL online od Ubuya?

Jednostavno je kupovati Tris-HCl Buffer, pH 7.0 [1M] - 250mL online s Ubuya.. Samo trebate potražiti proizvod, odabrati način dostave tijekom odjave i dobiti ga na svoju lokaciju.

Je li Tris-HCl Buffer, pH 7.0 [1M] - 250mL dostupan za online kupnju u Croatia?

Da, na Ubuyu Croatia ovaj proizvod vam je dostupan za kupnju po razumnoj cijeni.. Tris-HCl Buffer, pH 7.0 [1M] - 250mL nije dostupan lokalno, ali možete nam povjeriti naše usluge ekspresne dostave.

Koliko dugo je potrebno da dobijete proizvod nakon narudžbe?

Vrijeme isporuke vašeg naručenog proizvoda ovisi o tome što ste naručili i načinu dostave koji ste odabrali.. Predviđeno vrijeme dostave navedeno je tijekom procesa naplate, stoga budite bezbrižni prilikom kupovine.

eCon Lab Supply Pogledaj izvornik

Manufactured in an ISO 9001 and 13485 certified facility in the USA under stringent Good Manufacturing Practices.

eCon Lab Supply

Stavka #: 97963856

Tris-HCl Buffer, pH 7.0 [1M] - 250mL

Stavka #: 97963856

€ 132

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from SAD}

Dostupnost : Na lageru

Uvezeno iz USA trgovine

QTY:

Naručite sada i dobijte ga oko Četvrtak, Listopad 01

Secured transaction

Our Top Logistics Partners

Manufactured in an ISO 9001 and 13485 certified facility in the USA under stringent Good Manufacturing Practices.

U-Care jamstvo:

Nijedan

Odaberite plan

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of SAD store (110-120). Recommended power converters Kupi sada.

What Stands Out

Optimal pH Stability

This Tris-HCl buffer maintains a consistent pH of 7.0, ideal for biochemical assays and molecular biology applications, ensuring reliable experimental conditions and reproducible results.

High Concentration

At 1M concentration, this buffer solution offers a robust capability to resist pH changes, making it suitable for various intricate laboratory procedures involving sensitive reactions.

Convenient Volume Size

Available in a 250mL size, this buffer is perfect for regular laboratory use, minimizing waste while providing ample quantity for multiple experiments or applications.

Detalji o proizvodu

Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.42
Item Weight	1.8 pounds (820 grams)
Brand	eCon Lab Supply

Who Should Buy?

Biochemistry Students
Ideal for laboratory experiments involving enzyme reactions and protein studies at a controlled pH level.
Molecular Biology Research
Essential for applications like PCR, gel electrophoresis, and DNA/RNA manipulation requiring stable pH.
Clinical Labs
Useful in diagnostic tests that require precise buffer solutions for maintaining biological sample integrity.

Field Research
Not suitable for outdoor experiments due to stability issues and potential for buffer degradation over time.

OPIS PROIZVODA

Tris-HCl Buffer, pH 7.0 [1M] - 250mL

Imate li pitanja? Cavrljajte s nama

Pitanja i odgovori kupaca

pitanje: Kako kupovati Tris-HCl Buffer, pH 7.0 [1M] - 250mL online od Ubuya?

odgovor: Jednostavno je kupovati Tris-HCl Buffer, pH 7.0 [1M] - 250mL online s Ubuya.. Samo trebate potražiti proizvod, odabrati način dostave tijekom odjave i dobiti ga na svoju lokaciju.
pitanje: Je li Tris-HCl Buffer, pH 7.0 [1M] - 250mL dostupan za online kupnju u Croatia?

odgovor: Da, na Ubuyu Croatia ovaj proizvod vam je dostupan za kupnju po razumnoj cijeni.. Tris-HCl Buffer, pH 7.0 [1M] - 250mL nije dostupan lokalno, ali možete nam povjeriti naše usluge ekspresne dostave.
pitanje: Koliko dugo je potrebno da dobijete proizvod nakon narudžbe?

odgovor: Vrijeme isporuke vašeg naručenog proizvoda ovisi o tome što ste naručili i načinu dostave koji ste odabrali.. Predviđeno vrijeme dostave navedeno je tijekom procesa naplate, stoga budite bezbrižni prilikom kupovine.

eCon Lab Supply Buffers Editorial Review

Nije pronađena urednička recenzija

Customer Reviews & Ratings

5.0

1 ocjene kupaca

5 zvjezdica

100%
4 zvjezdica

0%
3 zvjezdica

0%
2 zvjezdica

0%
1 zvjezdica

0%

Recenzirajte ovaj proizvod

Podijelite svoje misli s drugim kupcima

Pros

Stable pH for experiments
Easy to use
High-quality formulation
Versatile for various applications
Trusted by researchers

Protiv

Bottle design could be improved.

Product Price History

Važne informacije

Ograničenja: imajte na umu da jamstvo možda ne vrijedi za proizvode koji se dostavljaju u inozemstvo; servis možda neće biti dostupan; priručnici proizvoda, upute i sigurnosna upozorenja možda nisu na jeziku zemlje odredišta; proizvodi (i prateći materijali) možda nisu u skladu sa standardima, specifikacijama i zahtjevima za označavanje zemlje odredišta; proizvod možda nije usklađen s naponom struje i drugim električnim standardima (zbog čega će možda trebati adapter ili pretvarač). Primatelj je odgovoran za provjeru može li proizvod prema važećim zakonima biti uvezen u zemlju odredišta. Kad naručujete od Ubuya ili njegovih partnera, primatelj se smatra registriranim uvoznikom i mora se pridržavati svih zakona i propisa zemlje odredišta.
Svi proizvodi navedeni na Ubuyu nisu za prodaju, jer je Ubuy globalna tražilica. Proizvodi podliježu propisima o izvozu i trgovini.

€ 132

Naručite sada i dobijte ga oko Četvrtak, Listopad 01

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

Značajke i prednosti

Comprehensive range of biological buffers for research and diagnostics.
Produced in an ISO certified facility ensuring quality.
Aseptically filtered for purity and reliability.
Suitable for protein research and molecular biology applications.
Complete Certificate of Analysis provided for each lot.
Dilutable Tris-HCl buffer for tailored concentration needs.

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

Tris-HCl Buffer, pH 7.0 [1M] - 250mL

€ 132

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Detalji o proizvodu

Who Should Buy?

OPIS PROIZVODA

Pitanja i odgovori kupaca

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

Recenzirajte ovaj proizvod

Pros

Protiv

Product Price History

Važne informacije

€ 132

Značajke i prednosti

Ubuy Assurance

Fast
Shipping

Free
Return^*