What is Tris buffer and what is its primary use?

Tris buffer, or tris(hydroxymethyl)aminomethane buffer, is widely used in biochemical and molecular biology applications to maintain a stable pH. Its primary use is in molecular biology techniques such as gel electrophoresis and protein purification. Maintaining consistent pH levels is crucial for the stability and functionality of various biomolecules during experiments.

Why is pH 8.0 important for Tris buffer?

A pH of 8.0 is significant for Tris buffer because it provides an optimal environment for many biological reactions, particularly those involving enzymes that function best in slightly alkaline conditions. This pH range helps maintain the solubility and stability of proteins and nucleic acids, ensuring accurate and reproducible experimental results.

How do you prepare a 1M Tris buffer solution?

To prepare a 1M Tris buffer solution at pH 8.0, dissolve 121.14 grams of Tris base in a sufficient volume of distilled water. Then, adjust the pH to 8.0 using hydrochloric acid (HCl) or phosphoric acid. Finally, dilute the solution to a final volume of 1000 mL with distilled water. This preparation allows for controlled pH adjustments suitable for a variety of research applications.

Can Tris buffer be used for DNA and RNA sample preparation?

Yes, Tris buffer is frequently used in the preparation of DNA and RNA samples. Its ability to maintain a stable pH ensures the integrity of nucleic acids during isolation and purification processes. Moreover, it helps to enhance the performance of enzymatic reactions such as polymerase chain reactions (PCR), where pH balance is critical for enzyme activity.

What are the storage requirements for Tris buffer?

Tris buffer should be stored at room temperature, away from direct sunlight. It is recommended to keep the buffer in a tightly sealed container to avoid contamination and evaporation. Stored correctly, Tris buffer can maintain its effectiveness for an extended period, making it ideal for regular laboratory use.

Is Tris buffer compatible with other reagents?

Tris buffer is generally compatible with most common laboratory reagents. However, compatibility may vary with specific chemicals, particularly those that are acid-sensitive. Always refer to the manufacturer's recommendations and conduct compatibility tests during experimental planning to ensure optimal results.

What are some common applications of Tris buffer in laboratory settings?

Tris buffer is commonly used in applications such as electrophoresis, western blotting, and DNA/RNA extraction and analysis. Its versatility makes it a staple in laboratory experiments, especially in molecular biology, biochemistry, and biophysical studies. Additionally, it's involved in buffer systems for various enzymes and reactions across scientific disciplines.

Can Tris buffer be used for cell culture?

While Tris buffer is not commonly used as a standalone buffer in cell culture, it can be effective in certain applications where pH stability is necessary. Researchers might incorporate Tris buffer for specific assays or treatments within a cell culture setup to ensure optimal pH levels during experiments.

How does Tris buffer affect protein stability?

Tris buffer plays a critical role in maintaining protein stability by creating an optimal pH environment. Proteins are sensitive to changes in pH, which can lead to denaturation and loss of function. Using Tris buffer helps preserve protein structure and activity during experiments, particularly those involving enzymatic assays or analytical techniques.

Where can I buy Tris Buffer, pH 8.0 [1M] - 1000mL Volume?

You can purchase Tris Buffer, pH 8.0 [1M] - 1000mL Volume from Ubuy. They offer a wide variety of laboratory supplies and consumables, ensuring you can find the right products for your research needs in Croatia.

Kako kupovati Tris Buffer, pH 8.0 [1M] - 1000mL Volume online od Ubuya?

Jednostavno je kupovati Tris Buffer, pH 8.0 [1M] - 1000mL Volume online s Ubuya.. Samo trebate potražiti proizvod, odabrati način dostave tijekom odjave i dobiti ga na svoju lokaciju.

Je li Tris Buffer, pH 8.0 [1M] - 1000mL Volume dostupan za online kupnju u Croatia?

Da, na Ubuyu Croatia ovaj proizvod vam je dostupan za kupnju po razumnoj cijeni.. Tris Buffer, pH 8.0 [1M] - 1000mL Volume nije dostupan lokalno, ali možete nam povjeriti naše usluge ekspresne dostave.

Koliko dugo je potrebno da dobijete proizvod nakon narudžbe?

Vrijeme isporuke vašeg naručenog proizvoda ovisi o tome što ste naručili i načinu dostave koji ste odabrali.. Predviđeno vrijeme dostave navedeno je tijekom procesa naplate, stoga budite bezbrižni prilikom kupovine.

eCon Lab Supply Pogledaj izvornik

Tris Buffer, pH 8.0 [1M] - 1000mL Volume

Manufactured in an ISO 9001 and 13485 certified facility in the USA, our 1M Tris Buffer pH 8.0 is essential for your molecular biology and proteomics applications.

eCon Lab Supply

Stavka #: 98743277

Tris Buffer, pH 8.0 [1M] - 1000mL Volume

Stavka #: 98743277

€ 49

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from SAD}

Dostupnost : Na lageru

Uvezeno iz USA trgovine

QTY:

Naručite sada i dobijte ga oko Srijeda, Rujan 02

Secured transaction

Our Top Logistics Partners

Manufactured in an ISO 9001 and 13485 certified facility in the USA, our 1M Tris Buffer pH 8.0 is essential for your molecular biology and proteomics applications.

U-Care jamstvo:

Nijedan

Odaberite plan

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of SAD store (110-120). Recommended power converters Kupi sada.

What Stands Out

High Purity

This Tris buffer offers superior purity, ensuring reliable and consistent results in biological and chemical applications, making it an essential reagent for researchers and laboratories.

Stable pH

Maintaining a stable pH of 8.0, this buffer effectively minimizes variations during experiments, which is crucial for reproducibility and accuracy in various biochemical assays.

Convenient Volume

Available in a convenient 1000mL volume, it provides ample quantity for extensive testing, reducing the need for frequent reordering and saving time for busy laboratories.

Detalji o proizvodu

Container Type	Bottle
Item Form	Liquid
Item Weight	1.3 Pounds (590 grams)
Volume	1000 Milliliters
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Part Number	23.105.32
Included Components	Bottle
Brand	eCon-Lab-Supply
Item Volume	1000 Milliliters
Package Information	Bottle

Who Should Buy?

Molecular Biologists
Ideal for DNA and RNA experiments, providing stable pH for enzymatic reactions and nucleic acid storage.
Biochemists
Useful for protein purification and analysis, ensuring appropriate buffering conditions for various biochemical assays.
Laboratories
Fits well in educational and research labs for standardizing pH in various experimental protocols efficiently.

Non-Scientific Users
Not suitable for casual users without a background in scientific applications or understanding of buffer systems.

OPIS PROIZVODA

Tris Buffer, pH 8.0 [1M] - 1000mL Volume

Imate li pitanja? Cavrljajte s nama

Pitanja i odgovori kupaca

pitanje: Kako kupovati Tris Buffer, pH 8.0 [1M] - 1000mL Volume online od Ubuya?

odgovor: Jednostavno je kupovati Tris Buffer, pH 8.0 [1M] - 1000mL Volume online s Ubuya.. Samo trebate potražiti proizvod, odabrati način dostave tijekom odjave i dobiti ga na svoju lokaciju.
pitanje: Je li Tris Buffer, pH 8.0 [1M] - 1000mL Volume dostupan za online kupnju u Croatia?

odgovor: Da, na Ubuyu Croatia ovaj proizvod vam je dostupan za kupnju po razumnoj cijeni.. Tris Buffer, pH 8.0 [1M] - 1000mL Volume nije dostupan lokalno, ali možete nam povjeriti naše usluge ekspresne dostave.
pitanje: Koliko dugo je potrebno da dobijete proizvod nakon narudžbe?

odgovor: Vrijeme isporuke vašeg naručenog proizvoda ovisi o tome što ste naručili i načinu dostave koji ste odabrali.. Predviđeno vrijeme dostave navedeno je tijekom procesa naplate, stoga budite bezbrižni prilikom kupovine.

eCon Lab Supply Buffers Editorial Review

Nije pronađena urednička recenzija

Customer Reviews & Ratings

4.0

1 ocjene kupaca

5 zvjezdica

0%
4 zvjezdica

100%
3 zvjezdica

0%
2 zvjezdica

0%
1 zvjezdica

0%

Recenzirajte ovaj proizvod

Podijelite svoje misli s drugim kupcima

Pros

Highly stable pH range
Wide application in biology labs
Easy to prepare and use
Cost-effective for large volumes
consistent results across experiments

Protiv

Limited shelf life after opening

Product Price History

Važne informacije

Ograničenja: imajte na umu da jamstvo možda ne vrijedi za proizvode koji se dostavljaju u inozemstvo; servis možda neće biti dostupan; priručnici proizvoda, upute i sigurnosna upozorenja možda nisu na jeziku zemlje odredišta; proizvodi (i prateći materijali) možda nisu u skladu sa standardima, specifikacijama i zahtjevima za označavanje zemlje odredišta; proizvod možda nije usklađen s naponom struje i drugim električnim standardima (zbog čega će možda trebati adapter ili pretvarač). Primatelj je odgovoran za provjeru može li proizvod prema važećim zakonima biti uvezen u zemlju odredišta. Kad naručujete od Ubuya ili njegovih partnera, primatelj se smatra registriranim uvoznikom i mora se pridržavati svih zakona i propisa zemlje odredišta.
Svi proizvodi navedeni na Ubuyu nisu za prodaju, jer je Ubuy globalna tražilica. Proizvodi podliježu propisima o izvozu i trgovini.

€ 49

Naručite sada i dobijte ga oko Srijeda, Rujan 02

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

Značajke i prednosti

Comprehensive range of biological buffers for research and diagnostics.
Manufactured in an ISO 9001 and 13485 certified facility.
Strictly adheres to Good Manufacturing Practices.
1M Tris Buffer pH 8.0 is ready-to-use and versatile.
Filtered sterilized for optimal purity and performance.
Suitable for all DNA and RNA applications.

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

Tris Buffer, pH 8.0 [1M] - 1000mL Volume

€ 49

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Detalji o proizvodu

Who Should Buy?

OPIS PROIZVODA

Pitanja i odgovori kupaca

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

Recenzirajte ovaj proizvod

Pros

Protiv

Product Price History

Važne informacije

€ 49

Značajke i prednosti

Ubuy Assurance

Fast
Shipping

Free
Return^*