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Stavka #:
97964044
50X TAE Buffer, 100 mL
Stavka #:
97964044
€ 42
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50X TAE Buffer protects DNA during electrophoresis, ensuring your results are reliable and accurate.
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Detalji o proizvodu
| Item Weight | 0.25 lbs (110 grams) |
OPIS PROIZVODA
50X TAE Buffer, 100 mL
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Pitanja i odgovori kupaca
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pitanje:
What is 50X TAE Buffer used for?
odgovor: 50X TAE Buffer is primarily used in molecular biology for the electrophoresis of nucleic acids. This buffer maintains a stable pH during the separation of DNA and RNA and provides essential ions that help in the migration of nucleic acids through gels. When diluted to 1X concentration, it is optimal for running agarose gels, making it crucial in applications such as gel electrophoresis and DNA fragment analysis. -
pitanje:
How do I dilute 50X TAE Buffer for use?
odgovor: To use 50X TAE Buffer, it needs to be diluted to 1X concentration before application. This is achieved by mixing 1 part of the 50X solution with 49 parts of distilled water. For example, if you need 100 mL of 1X TAE Buffer, combine 2 mL of 50X TAE Buffer with 98 mL of water. This dilution is vital for effective DNA separation and band visualization in gel electrophoresis. -
pitanje:
What is the shelf life of 50X TAE Buffer?
odgovor: The shelf life of 50X TAE Buffer varies depending on storage conditions, but generally, it can remain stable for extended periods if kept in a cool and dry place. To ensure optimal performance, always check for any turbidity, discoloration, or precipitation before use. Proper storage will help maintain its effectiveness for experiments that involve DNA manipulation and analysis, reducing the risk of experimental failure. -
pitanje:
Is 50X TAE Buffer compatible with agarose gel electrophoresis?
odgovor: Yes, 50X TAE Buffer is highly compatible with agarose gel electrophoresis. It provides the necessary ionic environment that stabilizes the DNA while separating it based on size. This buffer is preferred for applications requiring high resolution in DNA fragment size determination. When diluted appropriately to 1X, it ensures effective migration of DNA through the gel matrix, allowing for clear visualization of the bands. -
pitanje:
Can I use 50X TAE Buffer for RNA electrophoresis?
odgovor: Yes, 50X TAE Buffer can be used for RNA electrophoresis, but its use in RNA analysis should be approached with care. It is important to ensure that the RNA samples are not degraded, and the buffer is fresh. RNA is sensitive to degradation; therefore, using 50X TAE Buffer allows better resolution of RNA bands when diluted correctly, promoting accurate electrophoresis results. -
pitanje:
What are the components of 50X TAE Buffer?
odgovor: 50X TAE Buffer consists of Tris base, acetic acid, and EDTA (ethylenediaminetetraacetic acid). Tris acts as a buffering agent to maintain pH, acetic acid provides the acidic environment, and EDTA chelates divalent metal ions, which can otherwise interfere with DNA and enzymes. The combination creates a stable buffer system essential for nucleic acid studies, providing researchers with optimal conditions for their experiments. -
pitanje:
Is 50X TAE Buffer necessary for DNA gel extraction?
odgovor: 50X TAE Buffer is not strictly necessary for DNA gel extraction but is useful in maintaining the integrity of DNA post-electrophoresis. After running your gel, the buffer can help rehydrate the gel and provide a stable pH environment for further DNA processing. It can also be beneficial when preparing samples for downstream applications, allowing for better recovery of DNA fragments due to its compatibility with various procedures. -
pitanje:
How should I store 50X TAE Buffer?
odgovor: To preserve the stability of 50X TAE Buffer, it should be stored in a cool, dark place at room temperature or refrigerated. Make sure the container is sealed tightly to prevent contamination. Proper storage conditions help prevent degradation or changes in composition, ensuring reliable results during experiments involving nucleic acids, such as PCR preparation or gel electrophoresis. -
pitanje:
What are some common applications for using 50X TAE Buffer?
odgovor: 50X TAE Buffer has several applications, primarily in molecular biology, including gel electrophoresis for DNA and RNA, restriction enzyme digestion, and PCR preparations. It helps facilitate the migration of nucleic acids through gels, aiding in the quantification and analysis of DNA fragments. Researchers often use it for cloning experiments and during the preparation of samples for sequencing, ensuring high-quality results. -
pitanje:
Where can I buy 50X TAE Buffer, 100 mL?
odgovor: You can buy 50X TAE Buffer, 100 mL on Ubuy in Croatia. Ubuy provides a convenient online shopping platform where you can find various laboratory supplies, including different concentrations of TAE Buffer, ensuring you get the right product for your research needs.
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Pros
- High purity formula
- Convenient 100 mL size
- Well-suited for various applications
- Easy to store and use
- Reliable performance
Protiv
- Cap can be slightly hard to open.
Product Price History
Važne informacije
- Ograničenja: imajte na umu da jamstvo možda ne vrijedi za proizvode koji se dostavljaju u inozemstvo; servis možda neće biti dostupan; priručnici proizvoda, upute i sigurnosna upozorenja možda nisu na jeziku zemlje odredišta; proizvodi (i prateći materijali) možda nisu u skladu sa standardima, specifikacijama i zahtjevima za označavanje zemlje odredišta; proizvod možda nije usklađen s naponom struje i drugim električnim standardima (zbog čega će možda trebati adapter ili pretvarač). Primatelj je odgovoran za provjeru može li proizvod prema važećim zakonima biti uvezen u zemlju odredišta. Kad naručujete od Ubuya ili njegovih partnera, primatelj se smatra registriranim uvoznikom i mora se pridržavati svih zakona i propisa zemlje odredišta.
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€ 42
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Značajke i prednosti
- Contains 2M Tris, 1M Acetic acid, and 0.05M EDTA for effective nucleic acid electrophoresis.
- Inhibits nucleases to protect DNA integrity during experiments.
- Powers efficient running of both genomic and supercoiled DNA.
- Faster dsDNA run compared to TBE buffer, enhancing experiment efficiency.
- Manufactured under strict quality control in an ISO certified facility.
- Ideal for research purposes, compliant with Good Manufacturing Practices.