What is Lab Buffer, Hanks' Balanced Salt Solution used for?

Lab Buffer, specifically Hanks' Balanced Salt Solution (without calcium and magnesium), is primarily used in biological and microbiological laboratories. It serves as a nutrient solution that helps maintain the osmotic balance of cells in culture, making it ideal for use in cell and tissue culture applications. By providing essential ions and nutrients, it supports cellular functions, enabling researchers to study various biological processes effectively, such as growth, proliferation, and responses to treatments.

Why is calcium and magnesium omitted from Hanks' Balanced Salt Solution?

Calcium and magnesium are often omitted from specific formulations of Hanks' Balanced Salt Solution to meet particular experimental needs. In certain cell culture scenarios, the presence of these ions can interfere with experimental outcomes, such as cell adhesion or aggregation. Therefore, researchers can use the calcium and magnesium-free version when working with cell types sensitive to these ions, ensuring more controlled conditions and reliable results in experiments focused on cell behavior or pharmacological studies.

How should Lab Buffer, Hanks' Balanced Salt Solution be stored?

Lab Buffer should be stored in a cool, dry place, away from direct sunlight and extreme temperatures. Once opened, it’s best to keep it sealed properly to prevent contamination. Refrigeration can extend its shelf life, but ensure to bring it back to room temperature before use. Proper storage is crucial to maintain the integrity of the solution, ensuring it remains effective for supporting cell viability and health during culture.

Can Hanks' Balanced Salt Solution be used for washing cells?

Yes, Hanks' Balanced Salt Solution is commonly used for washing cells during various laboratory protocols. Its balanced ionic composition helps to rinse away residues of serum or other media components that may interfere with the experiment. This wash step is particularly important prior to trypsinization or any treatment applications, as it helps maintain optimal conditions for cell detachment and minimizes the risk of altered cell behavior.

Is Hanks' Balanced Salt Solution suitable for all cell types?

While Hanks' Balanced Salt Solution is versatile, it may not be suitable for every cell type. Most adherent cells thrive in this solution, but suspension cells or specific cell lines requiring growth factors may need a complete medium for optimal growth. It's essential to assess the requirements of your specific cell type and adapt the protocol accordingly. For specialized applications, researchers sometimes supplement Hanks' with additional nutrients or growth factors for enhanced performance.

What are the common applications of Hanks' Balanced Salt Solution in research?

Hanks' Balanced Salt Solution is widely used in various applications, including cell culture maintenance, washing cells before enzymatic treatment, and diluting reagents in assays. It’s an essential component in protocols involving the isolation of primary cells, transfection procedures, and metabolic studies. By maintaining an isotonic environment, it helps researchers study cellular responses to stimuli, drug effects, or environmental changes effectively.

How do I prepare Hanks' Balanced Salt Solution if I have the powdered version?

To prepare Hanks' Balanced Salt Solution from the powdered form, dissolve the recommended amount in distilled water, typically around 1 liter, while adjusting the pH to physiological levels (pH 7.2 to 7.4) using HCl or NaOH as needed. Once fully dissolved, filter the solution using a 0.22-micron filter to eliminate any particulates. This preparation allows for customized ionic strength and composition, ideal for specific experimental requirements.

What is the significance of isotonic solutions in cell culture?

Isotonic solutions, like Hanks' Balanced Salt Solution, are crucial for maintaining osmotic balance in cell culture. When cells are exposed to hypotonic or hypertonic environments, they can either swell and burst or shrink and become lysed, respectively, leading to cell death or compromised function. By using isotonic solutions, researchers ensure that cells retain their normal shape and physiological function, allowing for accurate experimental outcomes.

What kind of packaging does Lab Buffer, Hanks' Balanced Salt Solution come in?

Lab Buffer, Hanks' Balanced Salt Solution typically comes in sterile, sealed containers, often designed for laboratory use. Common packaging sizes include 1000ml bottles that are easy to handle and store. The packaging is designed to prevent contamination and degradation of the solution, ensuring that researchers can use the buffer safely for their assays and cell culture work without compromising quality.

Where can I buy Lab Buffer, Hanks' Balanced Salt Solution, Without Calcium & Magnesium, 1000ml?

You can buy Lab Buffer, Hanks' Balanced Salt Solution, Without Calcium & Magnesium, 1000ml from Ubuy in Croatia. Ubuy provides a user-friendly platform where you can find various laboratory products and chemicals, ensuring you have access to high-quality items that meet your research needs.

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Hanks' Balanced Salt Solution, HBSS Buffer Without Calcium & Magnesium, 0.1m Filtered Sterilization, Reay to Use Lab Buffer, 1000ml/ Bottles, 2 Bottles/Case

Hanks' Buffer is the most commonly used balanced salt solution in biological experiments.

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Hanks' Balanced Salt Solution, HBSS Buffer Without Calcium & Magnesium, 0.1m Filtered Sterilization, Reay to Use Lab Buffer, 1000ml/ Bottles, 2

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Calcium-Free Formula

This HBSS Buffer is free from calcium and magnesium, making it ideal for sensitive cell culture applications where these ions may interfere with results.

Filtered Sterilization

Each 1000ml bottle is filtered to 0.1 microns, ensuring a sterile solution that eliminates contaminants, promoting reliable experimental outcomes.

Ready to Use

Pre-prepared and packaged for immediate use, this buffer saves time in laboratory settings, allowing researchers to focus on experimentation instead of preparation.

Detalji o proizvodu

Brand Name	CryoKing
Unit Count	2 Count
Manufacturer	BIOLOGIX
Included Components	Hanks Balanced Salt Solution
Part Number	HANKS-1000-2-New
Brand	CryoKing
Item Weight	4 lbs (1.81 kg)

Who Should Buy?

Cell Culture
Ideal for researchers and labs conducting cell culture studies that require a stable environment without calcium and magnesium.
Tissue Preservation
Useful for laboratories needing a buffer to preserve tissues during handling and transportation for experiments.
Research Applications
Recommended for various biological experiments where a sterile, pH-balanced buffer solution is essential for accuracy.

Calcium-Magnesium Dependent Experiments
Not suitable for experiments that specifically require calcium and magnesium ions for proper biological reactions.

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Hanks' Balanced Salt Solution, HBSS Buffer Without Calcium & Magnesium, 0.1m Filtered Sterilization, Reay to Use Lab Buffer, 1000ml/ Bottles, 2 Bottles/Case

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Značajke i prednosti

No calcium or magnesium to maintain optimal trypsin activity.
Widely used for rinsing tissue blocks and cells in biological experiments.
User-friendly, ready-to-use solution for routine cell culture washing.
Low endotoxin levels due to advanced packaging for maximum purity.
Shelf life of 3 years, ensuring long-term reliability for research.
Ideal for anyone conducting ongoing biological experiments.

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Hanks' Balanced Salt Solution, HBSS Buffer Without Calcium & Magnesium, 0.1m Filtered Sterilization, Reay to Use Lab Buffer, 1000ml/ Bottles, 2

€ 47

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What Stands Out

Detalji o proizvodu

Who Should Buy?

OPIS PROIZVODA

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€ 47

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